Emulsion PCR: Techniques and applications (2025)

Kanagal-Shamanna, R. (2016). Emulsion PCR: Techniques and applications. In Methods in Molecular Biology (pp. 33-42). (Methods in Molecular Biology; Vol. 1392). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3360-0_4

Emulsion PCR: Techniques and applications. / Kanagal-Shamanna, Rashmi.
Methods in Molecular Biology. Humana Press Inc., 2016. p. 33-42 (Methods in Molecular Biology; Vol. 1392).

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Kanagal-Shamanna, R 2016, Emulsion PCR: Techniques and applications. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1392, Humana Press Inc., pp. 33-42. https://doi.org/10.1007/978-1-4939-3360-0_4

Kanagal-Shamanna R. Emulsion PCR: Techniques and applications. In Methods in Molecular Biology. Humana Press Inc. 2016. p. 33-42. (Methods in Molecular Biology). doi: 10.1007/978-1-4939-3360-0_4

Kanagal-Shamanna, Rashmi. / Emulsion PCR : Techniques and applications. Methods in Molecular Biology. Humana Press Inc., 2016. pp. 33-42 (Methods in Molecular Biology).

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title = "Emulsion PCR: Techniques and applications",

abstract = "Emulsion PCR (EmPCR) is a commonly employed method for template amplifi cation in multiple NGSbased sequencing platforms. The basic principle of emPCR is dilution and compartmentalization of template molecules in water droplets in a water-in-oil emulsion. Ideally, the dilution is to a degree where each droplet contains a single template molecule and functions as a micro-PCR reactor. Here, we discuss the basic principles, advantages, and challenges of applications of emPCR in clinical testing. We describe the methods of preparation and enrichment of template-positive Ion PGM{\texttrademark} Template OT2 200 Ion Sphere{\texttrademark} Particles (ISPs) on the Ion Personal Genome Machine {\textregistered} (PGM{\texttrademark}) System. For routine clinical testing, following library generation, we employ the automated Ion OneTouch{\texttrademark} System that includes the Ion OneTouch{\texttrademark} 2 and the Ion OneTouch{\texttrademark} ES instruments for template generation and enrichment of template- positive ISPs, respectively.",

keywords = "Emulsion PCR, Ion PGM{\texttrademark}, Ion sphere{\texttrademark} particles (ISPs)",

author = "Rashmi Kanagal-Shamanna",

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doi = "10.1007/978-1-4939-3360-0_4",

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N2 - Emulsion PCR (EmPCR) is a commonly employed method for template amplifi cation in multiple NGSbased sequencing platforms. The basic principle of emPCR is dilution and compartmentalization of template molecules in water droplets in a water-in-oil emulsion. Ideally, the dilution is to a degree where each droplet contains a single template molecule and functions as a micro-PCR reactor. Here, we discuss the basic principles, advantages, and challenges of applications of emPCR in clinical testing. We describe the methods of preparation and enrichment of template-positive Ion PGM™ Template OT2 200 Ion Sphere™ Particles (ISPs) on the Ion Personal Genome Machine ® (PGM™) System. For routine clinical testing, following library generation, we employ the automated Ion OneTouch™ System that includes the Ion OneTouch™ 2 and the Ion OneTouch™ ES instruments for template generation and enrichment of template- positive ISPs, respectively.

AB - Emulsion PCR (EmPCR) is a commonly employed method for template amplifi cation in multiple NGSbased sequencing platforms. The basic principle of emPCR is dilution and compartmentalization of template molecules in water droplets in a water-in-oil emulsion. Ideally, the dilution is to a degree where each droplet contains a single template molecule and functions as a micro-PCR reactor. Here, we discuss the basic principles, advantages, and challenges of applications of emPCR in clinical testing. We describe the methods of preparation and enrichment of template-positive Ion PGM™ Template OT2 200 Ion Sphere™ Particles (ISPs) on the Ion Personal Genome Machine ® (PGM™) System. For routine clinical testing, following library generation, we employ the automated Ion OneTouch™ System that includes the Ion OneTouch™ 2 and the Ion OneTouch™ ES instruments for template generation and enrichment of template- positive ISPs, respectively.

KW - Emulsion PCR

KW - Ion PGM™

KW - Ion sphere™ particles (ISPs)

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